Rapid determination of acetaminophen in plasma by LC-MS/MS / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 364-366, 2023.
Article
в Zh
| WPRIM
| ID: wpr-986014
Ответственная библиотека:
WPRO
ABSTRACT
Objective: To establish a method for the rapid determination of acetaminophen (APAP) in human plasma by LC-MS/MS. Methods: The plasma samples were extracted by methanol and acetonitrile (1: 1) and purified directly. C(18) column was used for sample separation. The mobile phase were methanol (5 mmol/L ammonium acetate) and water (5 mmol/L ammonium acetate). Samples were analyzed by LC MS/MS with the electrospray ionization multi reaction monitoring (MRM) mode. Results: The calibration curves of APAP was linear in the concentration range of 0~10 mg/L, the correlation coefficient (r) was greater than 0.999 0. The relative standard deviation within and between batches was less than 10%. The recovery rate were 96.81%~101.7%. The detection limit of the method was 0.1 μg/L and the lower limit of quantification was 0.3 μg/L. Conclusion: This method has strong specificity, high sensitivity and reliable determination results. It is suitable for the rapid analysis of clinical plasma samples.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Chromatography, High Pressure Liquid
/
Chromatography, Liquid
/
Methanol
/
Tandem Mass Spectrometry
/
Acetaminophen
Пределы темы:
Humans
Язык:
Zh
Журнал:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Год:
2023
Тип:
Article