Identification of High-affinity Novel Targeted Peptides for the Treatment of Human Ovarian Cancer
Prensa méd. argent
; 105(11): 816-826, dic2019. fig, tab, graf
Article
在 En
| LILACS, BINACIS
| ID: biblio-1049970
Responsible library:
AR392.1
ABSTRACT
Background:
To investigate the potential of the phage display-identified tumor cellbinding peptide as a biomarker of epithelial ovarian cancer using phage display technology.Method:
The Ph.D.-7 Phage Display Peptide Library was used to identify the specific conjugated phages with SKOV3 epithelial ovarian cancer cells, while Chinese hamster ovary cells formed the basis. After employing the rapid differential screening method invitro, the enzyme-linked immunosorbent assay (ELISA), DNA sequencing, immunohistochemistry, immunofluorescence, and the competitive inhibition test of synthetic peptides were used to determine the affinity and specificity of the phages with SKOV3 cells.Results:
Using bio panning, we screened the phages, showing a 3590-fold increase after the third round. A total of 61 titers of the phage were randomly selected for ELISA and 10 kinds of the phages with an optical density >0.5 were used for DNA sequencing. Clones of the phage TRRNIPN were derived from DNA sequencing based on ELISA, exhibiting both the brown granular phenomenon and green fluorescence. The specific targeted peptide TRRNIPN was incorporated in tumor cells through the competitive inhibition test.Conclusion:
The results of our study indicate that the phage display identified polypeptide TRRNIPN may be an effective biomarker for the early diagnosis and targeted therapy of ovarian cancerKey words
索引:
LILACS
主要主题:
Bacteriophages
/
DNA
/
Enzyme-Linked Immunosorbent Assay
/
Biomarkers, Tumor
/
Mass Screening
/
Peptide Library
/
Early Diagnosis
/
Research Report
研究类型:
Diagnostic_studies
/
Screening_studies
限制:
Female
/
Humans
语言:
En
期刊:
Prensa méd. argent
期刊主题:
MEDICINA
年:
2019
类型:
Article