Your browser doesn't support javascript.
loading
Iranian Journal of Public Health. 2005; 34 (1): 13-18
在 En | IMEMR | ID: emr-71103
Responsible library: EMRO
ABSTRACT
Cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite Cryptosporidium parvum. Water-borne transmission of this organism has become more prevalent in recent years. Current method for detection of C. parvum oocysts in water is immunofluoresence assay [IFA]. The method is time consuming, laborious and particularly not-specific. It cannot determine the infectivity of detected oocysts. We have evaluated a nested- PCR assay for sensitive detection of C. parvum oocysts in water samples. Water sample concentrates were spiked with Cryptosporidium oocysts and after DNA extraction and purification by QIAamp DNA mini kit, detection was achieved by nested PCR amplification of a 200 bp region of hsp70 gene specific for C. Parvum. The method could detect as few as one oocyst in seeded tap water samples. On the basis of these results, PCR could be a useful tool in the monitoring of water samples for the detection of Cryptosporidium oocysts
Subject(s)
在谷歌搜索
索引: IMEMR 主要主题: Virulence / Water Pollutants / DNA / Polymerase Chain Reaction / Fluorescent Antibody Technique / Cryptosporidiosis / Oocysts / Eukaryota 语言: En 期刊: Iran. J. Public Health 年: 2005
在谷歌搜索
索引: IMEMR 主要主题: Virulence / Water Pollutants / DNA / Polymerase Chain Reaction / Fluorescent Antibody Technique / Cryptosporidiosis / Oocysts / Eukaryota 语言: En 期刊: Iran. J. Public Health 年: 2005