A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Angelini, Leticia Mara Lima; Silva, Amanda Ribeiro Martins da; Rocco, Lucas de Freitas Coli; Milagre, Cintia Duarte de Freitas

Angelini, Leticia Mara Lima; Silva, Amanda Ribeiro Martins da; Rocco, Lucas de Freitas Coli; Milagre, Cintia Duarte de Freitas.

Affiliation

Angelini, Leticia Mara Lima; Universidade Estadual Paulista “Julio de Mesquita Filho”. Instituto de of Biociências. Rio Claro. BR
Silva, Amanda Ribeiro Martins da; Universidade Estadual Paulista “Julio de Mesquita Filho”. Instituto de of Biociências. Rio Claro. BR
Rocco, Lucas de Freitas Coli; Universidade Estadual Paulista “Julio de Mesquita Filho”. Instituto de of Biociências. Rio Claro. BR
Milagre, Cintia Duarte de Freitas; Universidade Estadual Paulista “Julio de Mesquita Filho”. Instituto de of Biociências. Rio Claro. BR

Braz. j. microbiol ; Braz. j. microbiol;46(1): 113-116, 05/2015. tab, graf

Article 在 En | LILACS | ID: lil-748237

Responsible library: BR1.1

ABSTRACT

ABSTRACT

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

Subject(s)
Key words

Amidase inhibitor; Amidase; High-throughput screening; Nitrilase; Nitrile hydratase

全文

加入你的收藏

打印

XML

在谷歌搜索

全文: 1 索引: LILACS 主要主题: Colorimetry / High-Throughput Screening Assays / Aminohydrolases / Hydro-Lyases 研究类型: Diagnostic_studies / Evaluation_studies / Screening_studies 语言: En 期刊: Braz. j. microbiol 期刊主题: MICROBIOLOGIA 年: 2015 类型: Article

全文

加入你的收藏

打印

XML

在谷歌搜索