Antitumor effect of cisplatin against murine ascites Dalton's lymphoma.

ABSTRACT

Cisplatin treatment brings about complete regression of ascites Dalton's lymphoma in mice. In the present study various steps involved during the tumor regression are examined using some biochemical and morphological parameters. During tumor regression, ascites fluid volume decreases sharply and there is an increase in carbohydrates and decrease in protein contents in the ascites supernatant after cisplatin treatment in vivo. Cisplatin treatment brings about definite changes in the arrangement/movement of surface membrane ruffles/blebs of tumor cells and causes infiltration of leukocytes (neutrophils, monocytes, lymphocytes) towards tumor cells. Membrane vesicles and vacuoles are also formed before the disintegration and lysis of tumor cells.