Detection of BCR-ABL Fusion Gene in Chronic Myeloid Leukemia by Novel Digital PCR / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1647-1656, 2023.
Article
在 Zh
| WPRIM
| ID: wpr-1010018
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish a new digital polymerase chain reaction (dPCR) system for the detection of BCR-ABL fusion gene in patients with chronic myeloid leukemia (CML), and explore its analytical performance and clinical applicability in the detection of BCR-ABLp190/210/230.@*METHODS@#A new dPCR system for detecting BCR-ABLp190/210/230 was successfully developed, and its sensitivity difference with qPCR and improvement of drug side effects in patients with CML during drug reduction or withdrawal were compared.@*RESULTS@#Among 176 samples, qPCR and dPCR showed high consistency in the sensitivity of detecting BCR-ABL (82.39%), and the positive rate of dPCR was about 5 times higher that of qPCR (20.45% vs 3.98%). During follow-up, blood routine (25% vs 10%), kidney/liver/stomach (25% vs 20%) and cardiac function (10% vs 0) were significantly improved after drug reduction or withdrawal in patients with initial dPCR negative compared with before drug reduction or withdrawal.@*CONCLUSIONS@#This new dPCR detection system can be applied to the detection of BCR-ABLp190/210/230. It has better consistency and higher positive detection rate than qPCR. Drug withdrawal or dose reduction guided by dPCR has a certain effect on improving drug side effects.
Key words
全文:
1
索引:
WPRIM
主要主题:
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Polymerase Chain Reaction
/
Fusion Proteins, bcr-abl
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Reverse Transcriptase Polymerase Chain Reaction
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Drug-Related Side Effects and Adverse Reactions
限制:
Humans
语言:
Zh
期刊:
Journal of Experimental Hematology
年:
2023
类型:
Article