ABSTRACT
AIM:
To explore the molecular mechanism of Tiaopi Chengqi decoction (TpCqD) improving
hyperthermia and high-
protein food-induced
hyperphagia mice based on
transcriptomics.
METHODS:
C57
mice were randomly divided into a
control group, model group, low-
dose TpCqD group, high-
dose TpCqD group, and
domperidone group. The general condition of the experimental
mice was observed and the average
food intake was counted, and the rate of
gastric emptying and intestinal propulsion was determined for each group of
mice. H&E
staining was used to observe pathological changes in gastric
tissue. PAS
staining was used to observe
glycogen changes in gastric
tissue.
Pepsin activity was determined by
colorimetry.
pH value of gastric contents was measured by
acid-base titration.
Transcriptome sequencing was used to analyze the differential
genes in gastric
tissue, a
volcano map and a cluster
heat map were made for the differential
genes, and KEGG was used to analyze the
signal pathway enrichment of the differential
genes. RT-qPCR verified the differential
genes obtained by
screening.
RESULTS:
After treatment with TpCqD, the
body weight and average
food intake of
mice with
food accumulation increased (P<0.05), and the intestinal propulsion rate and
gastric emptying speed of
mice with
food accumulation accelerated (P<0.05). TpCqD could protect gastric
tissue structure and
glycogen degradation, increase
pepsin activity (P<0.05), and reduce gastric content
pH (P<0.05).
Transcriptome results showed that TpCqD could regulate the expression of Acox2 and cilp2, regulate fat
digestion and
absorption,
protein digestion and
absorption, and pancreatic
secretion signals. RT-qPCR showed that compare with model group, TpCqD up-regulated Acox2 (P<0.05) and down-regulated the
mRNA level of cilp2 (P<0.05).
CONCLUSION:
TpCqD ameliorated digestive dysfunction in
mice with high-calorie and
high-protein diets leading to
food accumulation involving the
regulation of the fat and
sugar metabolism genes Acox2 and cilp2, and pancreatic secretory signaling.