ABSTRACT
To investigate the clinical
role of
T-cell transcription factor (TBX21) and
adenylate cyclase 9 antibody (ADCY9)
gene polymorphisms in the development of childhood
asthma.
METHODS:
Two hundred Han
Chinese wheezing children aged 5 years and younger in Henan region from July 2016 to January 2017 were selected as the study group, and another 100 Han
Chinese healthy
children aged 5 years and younger in the same period were selected as the
control group. Oral mucosal exfoliated
cells were collected from both groups, and the
genotypes of TBX21
gene rs2240017 polymorphic locus and ADCY9
gene rs2230739 polymorphic locus were detected by real-
time fluorescence quantitative
polymerase chain reaction (
PCR)
technique, and the
risk level of
asthma was assessed based on the test results. The
children in the low-
risk and high-
risk groups were compared in terms of
serum immunoglobulin E (
IgE) levels,
API positivity rate and allergic
disease incidence, and the correlation between the
risk level of
asthma-related
genetic polymorphisms and
serum IgE levels,
API and allergic
disease incidence was analyzed. All
children were followed up until 6 years of age to confirm the
diagnosis of
asthma, and the
incidence of
asthma was compared between the low-
risk and high-
risk groups.
Children with
asthma were treated with inhaled
glucocorticoids and
leukotriene receptor antagonists for 3 months, and the control of
asthma and the impairment of
lung function were compared between the low-
risk and high-
risk groups.
RESULTS:
The
genotype detection results of rs2240017 polymorphic locus of TBX21
gene and rs2230739 polymorphic locus of ADCY9
gene in the study group compared with those in the
control group were statistically significant (P<0.001). The percentages of CC, CT, and TT
genotypes of rs2240017 polymorphic locus of TBX21
gene were 19.50%, 56.00%, and 24.50%, respectively, and the percentages of CC, CG, and GG
genotypes of rs2230739 polymorphic locus of ADCY9
gene were 86.00%, 10.00%, and 4.00%, respectively, in 200
children with
wheezing;
serum IgE level,
API positivity rate and allergic
disease incidence were higher in the high-
risk group than in the low-
risk group (P< 0.001, <0.001, 0.021, respectively). The degree of
risk of
asthma-related
gene polymorphisms in
children with
wheezing was positively correlated with
serum IgE levels,
API positivity, and the
incidence of allergic
diseases (P<0.001); the
incidence of
asthma (81.48%) and impaired
lung function (74.07%) were higher in the high-
risk group than in the low-
risk group (4.90%, 3.50%) (P<0.001). There was no statistically significant difference between the
asthma control rate of
children with
asthma in the high-
risk group (79.55%) compared with the
asthma control rate of
children with
asthma in the low-
risk group (100.00%) (P=0.433).
CONCLUSION:
Gene polymorphisms at rs2240017 locus of TBX21
gene and rs2230739 locus of ADCY9
gene are closely associated with
asthma development and impaired
lung function in
children with
wheezing.