ABSTRACT
Objective:
To explore the effects of different
polymers on
in vitro biomimetic mineralization of small intestinal submucosa(SIS)scaffolds,and to evaluate the physicochemical properties and bio-compatibility of the SIS scaffolds.
Methods:
The SIS scaffolds prepared by freeze-drying
method were im-mersed in simulated
body fluid(SBF),mineralized liquid containing polyacrylic
acid(PAA)and mine-ralized liquid containing PAA and polyaspartic
acid(PASP).After two weeks in the mineralized solu-tion,the liquid was changed every other day.SBF@SIS,PAA@SIS,PAA/PASP@SIS scaffolds were ob-tained.The SIS scaffolds were used as
control group to evaluate their physicochemical properties and bio-compatibility.We observed the bulk morphology of the scaffolds in each group,analyzed the microscopic morphology by
environment scanning electron microscopy and determined the
porosity and pore size.We also analyzed the surface
elements by
energy dispersive X-ray spectroscopy(EDX),analyzed the struc-ture of functional groups by Flourier transformed infrared
spectroscopy(
FTIR),detected the
water ab-sorption rate by using
specific gravity method,and evaluated the compression strength by universal me-chanical testing machine.The pro-
cell proliferation effect of each group of scaffolds were evaluated by
CCK-8 cell proliferation method.
Results:
Under
scanning electron microscopy,the scaffolds of each group showed a three-dimensional porous structure with suitable pore size and
porosity,and crystal was observed in all the mineralized scaffolds of each group,in which the crystal deposition of PAA/PASP@SIS scaffolds was more regular.At the same
time,the
collagen fibers could be seen to thicken.EDX
analysis showed that the characteristic peaks of Ca and P were found in the three groups of mineralized scaffolds,and the highest peaks were found in the PAA/PASP@SIS scaffolds.
FTIR analysis proved that all the three groups of mineralized scaffolds were able to combine
hydroxyapatite with SIS.All the scaf-folds had good
hydrophilicity.The
compressive strength of the mineralized scaffold in the three groups was higher than that in the
control group,and the best
compressive strength was found in PAA/PASP@SIS scaffold.The scaffolds of all the groups could effectively adsorb
proteins,and PAA/PASP@SIS group had the best
adsorption capacity.In the
CCK-8 cell proliferation experiment,the PAA/PASP@SIS scaffold showed the best
ability to promote
cell proliferation with the largest number of living
cells observed.Con-clusionCompared with other mineralized scaffolds,PAA/PASP@SIS scaffolds prepared by mineralized
solution containing both PAA and PASP have better physicochemical properties and biocompatibility and have potential applications in
bone tissue engineering.
