ABSTRACT
Objective:
To study the regulatory mechanism of
p38 MAPK signaling pathway participate in hyperalge-sia reaction in
Parkinson's disease(PD)
rats model induced by 6-hydroxy
dopamine(
6-OHDA).
Methods:
Forty
male Sprague Dawley(SD)
rats were randomly divided into four groupsSham group(Sham),model group(
6-OHDA),
p38 MAPK inhibitor SB203580
treatment group(
6-OHDA+SB203580)and
p38 MAPK activator
anisomycin(ANS)
treatment group(
6-OHDA+ANS).PD model was established by intra-striatal
injection of
6-OHDA stereotactically.
6-OHDA+SB203580 and
6-OHDA+ANS groups was injected with
6-OHDA to establish PD model,and treated with inhibitor SB203580 or activator ANS respectively.The von Frey
hairs were applied to
measure the mechanical paw with-draw threshold(PWT)of
rats.
Enzyme linked immunosorbent assay(
ELISA)was used to detect the content of
IL-6,
IL-1β,and TNF-α in
rat dorsal root ganglion(
DRG).The
mRNA levels of
genes IL-6,
IL-1β,TNF-α,and
p38 MAPK in
rat DRG was detected by real
time RT-PCR.
Results:
In the
DRG of
6-OHDA included PD
rats,the expres-sion levels of
IL-6,
IL-1β,TNF-α,and
p38 MAPK were significantly increased(P＜0.05),and the PWT of
rats were significantly decreased(P＜0.05).The application of activator ANS further increased the expression levels of
IL-6,
IL-1β,TNF-α,and
p38 MAPK,and the PWT of
rats were decreased.After application of inhibitor SB203580,the ex-pression levels of
IL-6,
IL-1β,TNF-α and
p38 MAPK were significantly decreased in the
DRG of
rats(P＜0.05),and the PWT were significantly increased in
rats(P＜0.05).
Conclusion:
6-OHDA induces
mechanical hyperalgesia reaction in
rats,and the molecular mechanism is related to activation of the
p38 MAPK signalling pathway.
