Effect of Silibinin on Proliferation and Invasion of HeLa Cells and Its Mechanism / 华中科技大学学报(医学版)

ABSTRACT

Objective To investigate the effect of silibinin(SB)on the proliferation and invasion of cervical cancer HeLa cells and its mechanism.Methods CCK-8 was used to detect the effect of different concentrations of SB on the proliferation of HeLa cells,and the proper concentration of SB was screened.The cells were divided into 9 groupscontrol,SB-L(SB low dose),SB-M(SB medium dose),SB-H(SB high dose),PD98059(ERK1/2 signal inhibitor),SB202190(p38 MAPK inhibitor),PD98059+SB,SB202190+SB and cisplatin group.CCK-8 was used to detect cell proliferation.Transwell chamber was used to observe the cell invasion.Real-time PCR was used to detect the mRNA expression of matrix metalloproteinase-2(MMP-2),MMP-9,p-extracellular signal-regulated kinase(ERK)and p-p38.Western blot was used to detect the expression of MMP-2,MMP-9,ERK1/2,p38,p-ERK1/2 and p-p38.Results Compared with the control group,the proliferation rate and the number of inva-sive cells in other groups were significantly reduced(P＜0.05).And the expression levels of MMP-2,MMP-9,p-ERK1/2 and p-p38 were significantly decreased(P＜0.05).Compared with SB-M group,the above changes in PD98059+SB group and SB202190+SB group were more significant(P＜0.05).Conclusion SB can inhibit the proliferation and invasion of HeLa cells,and its mechanism may be related to inhibition of ERK/p38 MAPK signal pathway activation and downregulation of MMP-2 and MMP-9 expression.