ABSTRACT
BACKGROUND:
As
tissue engineering brings new
hope to the worldwide problem of
articular cartilage repair,the
construction of
light-curing 3D printed
hydrogel scaffolds with
biomimetic composition is of great significance for
cartilage tissue engineering.
OBJECTIVE:
To construct a
biomimetic methacryloylated
hyaluronic acid/acellular
Wharton's jelly composite
hydrogel scaffold by digital
light processing
3D printing technology,and to evaluate its biocompatibility.
METHODS:
Wharton's jelly was isolated and extracted from
human umbilical cord,then decellulated,freeze-dried,ground into
powder,and dissolved in PBS to prepare 50 g/L acellular
Wharton's jelly solution.Methylallylated
hyaluronic acid was prepared,lyophilized and dissolved in PBS to prepare 50 g/L methylallylated
hyaluronic acid solution.Acellular
Wharton's jelly solution was mixed with methacrylyacylated
hyaluronic acid solution at a volume ratio of 11,and was used as bio-
ink after adding photoinitiator.Methylacrylylated
hyaluronic acid hydrogel scaffolds(labeled as HAMA
hydrogel scaffolds)and methylacrylylated
hyaluronic acid/acellular
Wharton's jelly gel scaffolds(labeled as HAMA/WJ
hydrogel scaffolds)were prepared by digital
light processing
3D printing technology,and the microstructure,swelling performance,biocompatibility,and
cartilage differentiation performance of the scaffolds were characterized. RESULTS AND
CONCLUSION:
(1)Under scanning
electron microscope,the two groups of scaffolds showed a three-dimensional network structure,and the fiber connection of HAMA/WJ
hydrogel scaffold was more
uniform.Both groups achieved swelling equilibrium within 10 hours,and the equilibrium swelling ratio of HAMA/WJ
hydrogel scaffold was lower than that of HAMA
hydrogel scaffold(P＜0.05).(2)
CCK-8 assay showed that HAMA/WJ
hydrogel scaffold could promote the proliferation of
bone marrow mesenchymal stem cells compared with HAMA
hydrogel scaffold.Dead/live
staining showed that
bone marrow mesenchymal stem cells grew well on the two groups of scaffolds,and the
cells on the HAMA/WJ
hydrogel scaffolds were evenly distributed and more
cells were found.
Phalloidine staining showed better adhesion and spread of
bone marrow mesenchymal stem cells in HAMA/WJ
hydrogel scaffold than in HAMA.(3)
Bone marrow mesenchymal stem cells were inoculated into the two groups for chondrogenic induction
culture.The results of qRT-
PCR showed that the
mRNA expressions of agglutinoglycan,SOX9 and type Ⅱ
collagen in the HAMA/WJ
hydrogel scaffold group were higher than those in the HAMA
hydrogel scaffold group(P＜0.05,P＜0.01).(4)These findings indicate that the digital
light processing
3D bioprinting HAMA/WJ
hydrogel scaffold can promote the proliferation,adhesion,and chondrogenic differentiation of
bone marrow mesenchymal stem cells.
