Expression and Correlation of TRPV1/TRPM8 Channels in Intestinal and Spinal Dorsal Root Ganglia in Mice with Experimental Colitis / 医学研究杂志

ABSTRACT

Objective To investigate the expression of transient receptor potential vanilloid 1(TRPV1)and transient receptor po-tential melastatin member 8(TRPM8)in the intestinal and spinal dorsal root ganglion(DRG)in experimental colitis mice induced by dextran sodium sulfate(DSS),and further explore the connection and related pathways.Methods A total of 20male C57BL/6mice were selected and randomly divided into blank control group and DSS group,with 10mice in each group.Mice in the DSS group were treated with 30g/L DSS for 7days to construct colitis model.The hair,body mass,fecal characteristics(granular,loose stool,bloody stool,etc)and perianal conditions(redness,swelling,bloody stool,etc)of every group were observed and recorded at the same time every day,the colitis related disease activity index(DAI)calculated,and colon histopathological score were calculated according to the pathological sec-tions.The protein expression levels of TRPV1,TRPM8,Gαq in colon tissue were detected by Western blot.The expression levels of in-terleukin-6(IL-6),interleukin-1β(IL-1β)in the colon tissue were detected by real-time quantitative polymerase chain reaction(RT-PCR).The localization and expression of TRPV1,TRPM8,Gαq in colon tissue and DRG were detected by immunofluorescence.Results Compared with the blank control group,the colon pathological damage was obvious in the DSS group,and DAI score of colon tissue was significantly increased(P＜0.05),inflammatory factors including IL-1β,IL-6 and TRPV1,Gαq proteins were up-regula-ted significantly(P＜0.05),TRPM8 expression was down-regulated(P＜0.05).Meanwhile,co-expression of TRPV1/TRPM8,TR-PV 1/Gαq were observed in colonic mucosa and submucosa,and TRPV1/TRPM8 were co-expression in DRG.Compared with the blank control group,TRPV1 expression was increased and TRPM8 was decreased in DSS group.Conclusion The increased TRPV1 expres-sion and decreased TRPM8 expression are found in the colonic tissue of experimental colitis mice,and TRPV1/TRPM8 can be co-expressed in the colonic tissue and DRG.There may be some balance mechanism between them to maintain the stability of intestinal function.