Establishment of HPLC fingerprints and determination of eleven constituents for Qiangxin Mixture / 中成药
Chinese Traditional Patent Medicine
; (12): 3895-3901, 2023.
Article
在 Zh
| WPRIM
| ID: wpr-1028702
Responsible library:
WPRO
ABSTRACT
AIM To establish the HPLC fingerprints for Qiangxin Mixture,and to determine the contents of quercetin-3-O-β-D glucose 7-O-β-D gentil disglycoside(QGG),sinarin thiocyanate,erucinic acid,macrocetin,calycosin-7-glucoside,plananthine D,calycosin,baicalin,pilonidin,atracolactone Ⅲ and methylophiopogon dihydrogen isoflavone B.METHODS The analysis of methanol extract of this drug was performed on a 40℃thermostatic Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.01 mol/L phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS There were twenty common peaks in the fingerprints for fifteen batches of samples with the similarities of more than 0.930.Eleven constituents showed good linear relationships within their own ranges(r≥0.999 1),whose average recoveries were 98.47%-102.85%with the RSDs of 0.76%-1.74%.CONCLUSION This accurate and stable method can be used for the quality control of Qiangxin Mixture.