Intratumoral Administration of Secondary Lymphoid Chemokine and Unmethylated Cytosine-phosphorothioate-guanine Oligodeoxynucleotide Synergistically Inhibits Tumor Growth in Vivo
Journal of Korean Medical Science
; : 1270-1276, 2011.
Article
在 En
| WPRIM
| ID: wpr-127701
Responsible library:
WPRO
ABSTRACT
Secondary lymphoid tissue chemokine (SLC), which is expressed in T cell zones of secondary lymphoid organs, including the spleen and lymph nodes, strongly recruits both T lymphocytes and mature dendritic cells. As appropriate interaction of tumor-specific T cells and mature dendritic cells, equipped with tumor antigens, is a prerequisite for effective T cell immunity against established tumors, we mobilized lymphocytes and dendritic cells to tumor sites by intratumoral injection of secondary lymphoid tissue chemokine-Fc (SLC-Fc) fusion protein using the B16F10 murine melanoma model. Activation of dendritic cells, another prerequisite for the effective activation of naive tumor-specific T cells, was achieved by the addition of immunostimulatory cytosine-phosphorothioate-guanine oligodeoxynucleotide (CpG-ODN) into the tumor site. Intratumoral administration of SLC-Fc or CpG-ODN revealed antitumor effects against B16F10 murine melanoma grown in the subcutaneous space. Co-treatment of SLC-Fc and CpG-ODN displayed synergistic effects in reducing the tumor size. The synergistic antitumor effect in co-treatment group was correlated with the synergistic/additive increase in the infiltration of CD4+ T cells and CD11c+ dendritic cells in the tumor mass compared to the single treatment groups. These results suggest that the combined use of chemokines and adjuvant molecules may be a possible strategy in clinical tumor immunotherapy.
Key words
全文:
1
索引:
WPRIM
主要主题:
Oligodeoxyribonucleotides
/
Dendritic Cells
/
Melanoma, Experimental
/
T-Lymphocytes
/
CD4-Positive T-Lymphocytes
/
Injections, Intralesional
/
Chemotaxis, Leukocyte
/
CD11c Antigen
/
Cell Line, Tumor
/
Cell Proliferation
限制:
Animals
语言:
En
期刊:
Journal of Korean Medical Science
年:
2011
类型:
Article