Influence of the vitrification solution on the angiogenic factors in vitrificated mouse ovarian tissue
Obstetrics & Gynecology Science
; : 382-388, 2013.
Article
在 En
| WPRIM
| ID: wpr-17220
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To investigate the effect of the dimethyl sulfoxide (DMSO) and EFS-40 during vitrification on the expression of angiogenic factors in vitrified mouse ovarian tissue. METHODS: The ovarian tissues were obtained from 5 or 6 weeks aged ICR mouse. Ovarian tissues were divided into four groups: ovarian tissue without cryopreservation (control, group I), ovarian tissue vitrified with 15% DMSO (group II), ovarian tissue vitrified with EFS-40 (group III), and ovarian tissue slowly frozen with 10% DMSO (group IV). Thawing was carried out at room temperature. Levels of messenger RNA (mRNA) and protein for vascular endothelial growth factor-A (VEGF-A) and angiopoietin-2 (Angpt-2) were checked in ovarian tissues of four groups recovered on day 7 after cryopreservation. Reverse transcription-polymerase chain reaction and Western blot analysis were used to identify the levels of angiogenic factors in mouse ovarian tissues. RESULTS: Levels of mRNA and protein for VEGF-A and Angpt-2 were significantly decreased in cryopreserved group (group II, III and IV) than control group (group I) (P< 0.05). The significant differences of levels of mRNA and protein for VEGF-A and Angpt-2 between cryopreservation methods were observed (P< 0.05). Group III showed highest expression of mRNA and protein for VEFG-A and Angpt-2 than other cryopreservation groups (P< 0.05). CONCLUSION: These findings suggest that EFS-40 is more efficient vitrification solution for preservation of angiogenic factors than 15% DMSO during vitrification of mouse ovarian tissue. Future studies should investigate to improve the vitrification solution for ovarian tissue vitrification.
Key words
全文:
1
索引:
WPRIM
主要主题:
Ovary
/
RNA, Messenger
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Cryopreservation
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Dimethyl Sulfoxide
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Blotting, Western
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Reverse Transcriptase Polymerase Chain Reaction
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Angiopoietin-2
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Vascular Endothelial Growth Factor A
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Vitrification
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Methods
研究类型:
Prognostic_studies
限制:
Animals
语言:
En
期刊:
Obstetrics & Gynecology Science
年:
2013
类型:
Article