mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor beta2 in Adult Atopic Patients
Journal of Korean Medical Science
; : 1070-1074, 2006.
Article
在 En
| WPRIM
| ID: wpr-174099
Responsible library:
WPRO
ABSTRACT
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta2 subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta2 (IL-12R beta2) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta2 mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta2 mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta2 mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.
Key words
全文:
1
索引:
WPRIM
主要主题:
RNA, Messenger
/
Biomarkers
/
Reproducibility of Results
/
Risk Factors
/
Sensitivity and Specificity
/
RNA Editing
/
Risk Assessment
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Genetic Predisposition to Disease
/
Receptors, Interleukin-12
/
Hypersensitivity, Immediate
研究类型:
Diagnostic_studies
/
Etiology_studies
/
Risk_factors_studies
限制:
Adult
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Female
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Humans
/
Male
国家/地区名称主题:
Asia
语言:
En
期刊:
Journal of Korean Medical Science
年:
2006
类型:
Article