Recombinant influenza viruses as delivery vectors for hepatis B virus epitopes
Clinical and Experimental Vaccine Research
; : 77-82, 2012.
Article
在 En
| WPRIM
| ID: wpr-192289
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Neuraminidase (NA) of influenza virus contains stalk region that shows a great deal of variability in both amino acid sequence and length. In this paper, we investigated generation of recombinant influenza viruses that had hepatitis B virus (HBV) B cell epitopes in the NA stalk region as a dual vaccine candidate. MATERIALS ANDMETHODS:
We used the WSH-HK reassortant helper virus for rescue of recombinant influenza virus containing HBV epitopes and reverse genetic protocol based on the use of micrococcal nuclease-treated virus cores for reconstitution of ribonucleoproteins.RESULTS:
We successfully generated a chimeric influenza viruses which contained 22 amino acid peptides in the stalk region derived from the surface and pre-surface protein HBV. The growth kinetics of the recombinant viruses was investigated after infection of Madin-Darby canine kidney (MDCK) and Madin-Darby bovine kidney (MDBK) cells and the rIV-BVPreS virus showed higher titer than other viruses in MDCK cells. We also confirmed the presence of HBV epitopes in the chimeric viruses by enzyme-linked immunosorbent assay (ELISA) using anti-HBV polyclonal antibody. When the ratio of recombinant virus verse wild type virus was calculated by ELISA, recombinant viruses exhibited 2 fold higher values than the wild type virus.CONCLUSION:
These results suggest that chimeric influenza virus which contained foreign antigens can be used as dual vaccine against both HBV and influenza viruses.Key words
全文:
1
索引:
WPRIM
主要主题:
Orthomyxoviridae
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Peptides
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Viruses
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Enzyme-Linked Immunosorbent Assay
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Kinetics
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Hepatitis B virus
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Amino Acid Sequence
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Herpesvirus 1, Cercopithecine
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Epitopes, B-Lymphocyte
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Influenza, Human
语言:
En
期刊:
Clinical and Experimental Vaccine Research
年:
2012
类型:
Article