ABSTRACT
BACKGROUND:
Laminin, which is present in all
basement membrane, is a multifunctional
glycoprotein with diverse
biological activities. Like
fibronectin, it can influence
cell adhesion,
growth, morphology, differentiation, and migration as well as the assembly of the
extracellular matrix.
Laminin primarily
affects cells of epithelial origin, and response varies depending on the
cells.
OBJECTIVE:
Studies about the relationship of
laminin and the pathogenesis or
metastasis of
squamous cell carcinoma(SCC). MEHTODS In this study, we have examined the
laminin B1
mRNA expression and localizing pattern of
laminin in SCC, using Northern and slot-blot
hybridization, in situ hybridization and immunoperoxidase
stain.
RESULTS:
In
Northern blot hybridization, 5.6Kb
mRNA transcript was detected in control and SCC specimens. Slot-blot hybridization indicated theat amounts of
laminin B1
mRNA expression in SCC was 677.0+/-268.5 densitometer absorbance unit, in control was 228.0+/-16.37 unit respectively. There is a 3-fold increase in SCC compared to control. In
in situ hybridization, 35S-labeled
laminin B1
cDNA-
mRNA hybrid granules were detected along the
basement membrane and adjacent
cells of SCC. In immunoperoxidase
stain,
laminin has stained strongly and continuously along the
basement membrane of the
tumor nest in well differentiated SCC, and weakly and interrupted in poorly differentiated SCC.
CONCLUSION:
These results suggested that
laminin might be related to the pathogenesisand
metastasis of
squamous cell carcinomas.
