Alterations in NK Cell Cytotoxicity Induced by Peripheral Nerve Injury in Mice / 대한마취과학회지
Korean Journal of Anesthesiology
; : 219-223, 2007.
Article
在 Ko
| WPRIM
| ID: wpr-218005
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Chronic pain is often associated with changes in the immune responses, which highlights the need for the aggressive pain control to obtain a better prognosis. This study examined splenic NK cell cytotoxicity in an attempt to assess the possible changes in the immune function under chronic neuropathic pain after a partial transsection of the sciatic nerve. METHODS: After confirming tactile allodynia in response to the von Frey filament, a modified lactate dehydrogenase (LDH) release assay was used to determine the cytotoxic activity of splenic NK cells on the YAC-1 cell line in C3H/HeN (H-2k) mice (n = 6). NK cells as effector cells were mixed with YAC-1 cells as target cells (1 x 10(4)/100microliter), resulting in an effector-target ratio of 1 : 25, 1 : 50, 1 : 100 in the culture medium. RESULTS: At 1 and 2 weeks after the nerve injury, all the subjects showed significant mechanical sensitivity compared with those observed before surgery. The percentage of NK cell cytotoxicity of the neuropathic mice increased significantly 1 week after the nerve injury but decreased within 2 weeks compared with the normal mice. CONCLUSIONS: In terms of the altered NK cell cytotoxicity, neuropathic pain can cause changes in the normal performance of the immune function.
Key words
全文:
1
索引:
WPRIM
主要主题:
Peripheral Nerves
/
Prognosis
/
Sciatic Nerve
/
Cytotoxicity Tests, Immunologic
/
Killer Cells, Natural
/
Cell Line
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Chronic Pain
/
Peripheral Nerve Injuries
/
Hyperalgesia
/
Immune System
研究类型:
Prognostic_studies
限制:
Animals
语言:
Ko
期刊:
Korean Journal of Anesthesiology
年:
2007
类型:
Article