Ex vivo generation of functional dendritic cells from mobilized CD34+ hematopoietic stem cells
Yonsei Medical Journal
; : 328-338, 1998.
Article
在 En
| WPRIM
| ID: wpr-229298
Responsible library:
WPRO
ABSTRACT
The ability to generate dendritic cells (DCs) in sizeable numbers has enormous implications for the development of clinically-effective antigen presentation procedures for cancer immunotherapy. We evaluated the generation of immunostimulatory DCs from peripheral blood CD34+ cells collected from healthy donors. CD34+ cells purified from leukapheresis product were seeded at 1 x 10(4) cells/mL in complete medium supplemented with GM-CSF, TNF alpha, IL-4, c-kit ligand, and flt3 ligand (FL). By day 14 of culture in the presence of GM-CSF + TNF alpha, the total cell number increased by 23.4 +/- 5.4-fold compared to the starting number of CD34+ cells. When the c-kit and FL were added to GM-CSF and TNF alpha, the cell number increased by 109.8 +/- 11.2-fold without affecting the immunophenotype of recovered cells. Flow cytometric analysis indicated that cells with the markers of mature dendritic cells, i.e., CD1a +CD14 -HLA-DR+, and CD80+CD86+HLA-DR+, constituted 49.0% +/- 7.5%, and 38.9% +/- 6.5%, respectively. This pattern of expression of surface antigen was unchanged whether the c-kit ligand and/or FL was added. The irradiated CD1a+HLA-DR+ cells recovered from in vitro cultures elicit a vigorous proliferation of allogeneic peripheral blood T-cells, irrespective of cytokine combinations. These findings provide advantageous tools for the large-scale generation of DCs that are potentially usable for clinical protocols of immunotherapy or vaccination in patients undergoing cancer treatment.
Key words
全文:
1
索引:
WPRIM
主要主题:
Dendritic Cells
/
Hematopoietic Stem Cells
/
HLA-DR Antigens
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Granulocyte-Macrophage Colony-Stimulating Factor
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Interleukin-4
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Tumor Necrosis Factor-alpha
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Antigens, CD34
研究类型:
Guideline
限制:
Humans
语言:
En
期刊:
Yonsei Medical Journal
年:
1998
类型:
Article