Equol protects PC12 neuronal cells against hypoxia/reoxygenation injury in vitro by reducing reactive oxygen species production / 南方医科大学学报
Journal of Southern Medical University
; (12): 1-7, 2016.
Article
在 En
| WPRIM
| ID: wpr-232520
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Both of gp91(phox) (an isoform of nicotinamide adenine dinucleotide phosphate-reduced oxidases) and Src (a non-receptor protein tyrosine kinase) play a prominent role in mediating hypoxia/reoxygenation injury of neurons. The present study was designed to investigate the neuroprotective effect of equol, a predominant active metabolite of daidzein, against hypoxia/reoxygenation injury in rat pheochromocytoma cell line (PC12) and explore the underlying mechanisms.</p><p><b>METHODS</b>PC12 cells exposed to hypoxia/reoxygenation injury were examined for reactive oxygen species (ROS) using dihydroethidium and 2', 7'-dichlorofluorescein diacetate and analyzed for changes in lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) content. The expression levels of gp91(phox) and phosphorylated Src-Tyr416 (p-Src) were measured using Western blotting.</p><p><b>RESULTS</b>Equol dose-dependently restored the cell viability and decreased LDH activity and MDA content in culture medium of PC12 cells exposed to hypoxia/reoxygenation. Pretreatment of the cells with 10(-5) and 10(-6) mol/L equol inhibited hypoxia/reoxygenation-induced increase of ROS. PC12 cells treated with equol prior to hypoxia/reoxygenation injury showed significant enhancement of the protein levels of gp91(phox) and p-Src.</p><p><b>CONCLUSION</b>Equol confers neuroprotection against hypoxia/reoxygenation injury in PC12 cells by inhibiting the generation of ROS very likely as a result of down-regulation of gp91(phox) and inhibition of Src phosphorylation.</p>
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
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Phosphorylation
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Membrane Glycoproteins
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Cell Hypoxia
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Down-Regulation
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Cell Survival
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PC12 Cells
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Reactive Oxygen Species
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Neuroprotective Agents
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Src-Family Kinases
限制:
Animals
语言:
En
期刊:
Journal of Southern Medical University
年:
2016
类型:
Article