Association of abnormal cyclooxygenase-2 gene expression with colorectal carcinoma metastasis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.</p><p><b>METHODS</b>SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.</p><p><b>RESULTS</b>SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.</p><p><b>CONCLUSION</b>Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.</p>