Detection of bcr/abl fusion gene changes in patients with chronic myeloid leukemia after allo-HSCT by real-time quantitative reverse transcription polymerase chain reaction and its significance / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1350-1353, 2008.
Article
在 Zh
| WPRIM
| ID: wpr-234235
Responsible library:
WPRO
ABSTRACT
This study was aimed to detect the changes of bcr/able gene level in ph+ CML patients at different stages after allo-HSCT by real-time quantitative PCR and to evaluate the significance of this detection. The serial detection of bcr/abl fusion gene levels in 21 cases of CML treated with allo-HSCT was performed by RQ-PCR. The results showed that the bcr/able fusion gene could not be detected in 7 out 21 CML cases with positive fusion gene after allo-HSCT, while the bcr/abl fusion gene of different levels could be detected in 14 cases within 1-6 months. Dynamic detection indicated that the bcr/abl fusion gene levels in 9 cases were lower with relative value 0.0074%-0.088% and then could not be detected within 3-7 months after allo-HSCT. The bcr/abl fusion gene levels in 5 cases diagnosed as molecular relapse were between 0.077%-75%. The bcr/abl fusion gene levels in 1 out of 5 cases were 0.95%, 1.5%, and 0.16% in month 1, 2 and 3, respectively, and turned to negative in the month 4 without any treatment after allo-HSCT. 2 cases received the donor peripheral blood stem cell infusion, and then their bcr/abl mRNA levels could not be detected in bone marrow. Another 2 cases developed to the hematologic relapse, 1 out of 2 cases reached CR again after infusion of donor peripheral blood stem cells and chemotherapy, the other one died. It is concluded that serial quantifications of bcr/abl mRNA levels by RQ-PCR are reliable and can be used to detect the MRD, to monitor the outcome and to predict the relapse.
全文:
1
索引:
WPRIM
主要主题:
General Surgery
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Genes, abl
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Fusion Proteins, bcr-abl
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Hematopoietic Stem Cell Transplantation
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Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
研究类型:
Diagnostic_studies
/
Prognostic_studies
限制:
Adolescent
/
Adult
/
Female
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Humans
/
Male
语言:
Zh
期刊:
Journal of Experimental Hematology
年:
2008
类型:
Article