Zinc finger protein-activating transcription factor up-regulates vascular endothelial growth factor-a expression in vitro / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 171-175, 2012.
Article
在 En
| WPRIM
| ID: wpr-243243
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells.</p><p><b>METHODS</b>Firstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants.</p><p><b>RESULTS</b>The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamH1and Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg÷μL, P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165.</p><p><b>CONCLUSION</b>ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.</p>
全文:
1
索引:
WPRIM
主要主题:
Physiology
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Plasmids
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Molecular Sequence Data
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Base Sequence
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Up-Regulation
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Cells, Cultured
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Zinc Fingers
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Amino Acid Sequence
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Neovascularization, Physiologic
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Endothelial Cells
限制:
Humans
语言:
En
期刊:
Chinese Medical Sciences Journal
年:
2012
类型:
Article