Detection and typing assay of norovirus in acute hospitalizations among children less than 5 years old from 2008 to 2009 in Lulong, Hebei province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 11-13, 2012.
Article
在 Zh
| WPRIM
| ID: wpr-246196
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular epidemiologic characteristics and genotypes of norovirus in children less than 5 years of age in Lulong area from 2008 to 2009.</p><p><b>METHODS</b>325 stool specimens and epidemiological data from hospitalized children with diarrhea less than 5 years of age were collected. Rotavirus was detected by using the ELISA kit. Norovirus, adenovirus and astrovirus were detected by multiple reverse transcription-polymerase chain reaction (RT-PCR). Partial norovirus strains were sequenced and the tree was conducted by using the phylogenetic analyses.</p><p><b>RESULTS</b>Norovirus was detected in 37 out of 325 (11.3%) specimens,ranked only second to rotavirus (48.6%), and higher than adenovirus (6.5%) and astrovirus (4.3%). Norovirus predominantly infected children less than 2 years of age and the season peak of norovirus occurred in November. Phylogenetic analysis showed that the predominant strain was the GII. 4/2006b variant. Interestingly, a novel unreported GII-4 variant was found in this study.</p><p><b>CONCLUSION</b>Norovirus was one of the most important pathogens causing acute gastroenteritis from 2008 to 2009 in Lulong area. The GII. 4/2006b vairant was still the predominant strain. It is important to keep on monitoring the novel GII. 4 variant.</p>
全文:
1
索引:
WPRIM
主要主题:
Phylogeny
/
Time Factors
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Enzyme-Linked Immunosorbent Assay
/
China
/
Acute Disease
/
Epidemiology
/
Classification
/
Reverse Transcriptase Polymerase Chain Reaction
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Norovirus
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Hospitalization
研究类型:
Diagnostic_studies
限制:
Child, preschool
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Female
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Humans
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Infant
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Male
/
Newborn
国家/地区名称主题:
Asia
语言:
Zh
期刊:
Chinese Journal of Experimental and Clinical Virology
年:
2012
类型:
Article