Pleiotropic effect of tatC mutation on metabolism of pathogen Yersinia enterocolitica / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 445-449, 2007.
Article
在 En
| WPRIM
| ID: wpr-249828
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the impact of depletion of the twin arginine translocation (TAT) system on virulence and physiology of Yersinia enterocolitica for a better understanding of its pathogenicity.</p><p><b>METHODS</b>We constructed a DeltatatCSpR mutant of Yersinia enterocolitica by P1 phage mediated transduction using Escherichia coli K-12 DeltatatCSpR strain as a donor.</p><p><b>RESULTS</b>A P1-mediated genetic material transfer was found between the two species of enterobacteria, indicating a great potential of acquisition of antibiotic resistance in emergency of a new threatening pathogen by genetic material exchanges. Periplasmic trimethylamine N-oxidase reductase activity was detected in the wild type Y. enterocolitica strain and translocation of this enzyme was completely abolished by the DeltatatCSpR mutation. In addition, the DeltatatCSpR mutation showed a pleiotropic effect on the metabolism of Y. enterocolitica. However, the tat mutation did not seem to affect the mobility and virulence of Y. enterocolitica under the conditions used.</p><p><b>CONCLUSION</b>Unlike other pathogenic bacteria studied, the TAT system of Y. enterocolitica might play an important role in the pathogenic process, which is distinct from other pathogens, such as Pseudomonas aeruginosa and enterohemorrhagic E. coli O157H7.</p>
全文:
1
索引:
WPRIM
主要主题:
Transduction, Genetic
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Virulence
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Yersinia enterocolitica
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Drug Resistance, Microbial
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Oxidoreductases Acting on CH-NH Group Donors
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Genes, Bacterial
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Genetics
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Metabolism
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Mutation
语言:
En
期刊:
Biomedical and Environmental Sciences
年:
2007
类型:
Article