The Expression of TGF-beta Isoform mRNA in the Cataract Lens Epithelial Cell
Journal of the Korean Ophthalmological Society
; : 1393-1400, 2005.
Article
在 Ko
| WPRIM
| ID: wpr-25011
Responsible library:
WPRO
ABSTRACT
PURPOSE: Transforming growth factor (TGF)-beta is recognized as being associated with cataractogenesis. We quantitated the mRNA expression of TGF-beta isoforms in cataractous crystalline lens to determine the effect of the isoforms on cataractogenesis. METHODS: With lens epithelial cells from thirty eyes in thirty patients, the mRNA expressions of TGF-beta1, beta2 and beta3 were quantitated by real-time SYBR Green polymerase chain reaction and the results were compared according to cataract type and presence of diabetes mellitus. RESULTS: Each isoform mRNA of TGF-beta was expressed: TGF-beta3 in all 30 eyes, TGF-beta1 in 29 eyes (96.7%), with the exception being one diabetic senile cataract, and TGF-beta2 in 9 eyes. The amount of TGF-beta1 mRNA expression was significantly lower in the diabetic cataracts than in the non-diabetic cataracts (P=0.056). CONCLUSIONS: TGF-beta was associated with cataractogenesis. It is significant that the expression of TGF-beta2 mRNA was decreased in all cataracts. The decrease of TGF-beta1 mRNA expression was more meaningful in the diabetic cataracts than in the non-diabetic cataracts.
Key words
全文:
1
索引:
WPRIM
主要主题:
Cataract
/
RNA, Messenger
/
Transforming Growth Factors
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Polymerase Chain Reaction
/
Transforming Growth Factor beta
/
Protein Isoforms
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Diabetes Mellitus
/
Epithelial Cells
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Transforming Growth Factor beta1
/
Transforming Growth Factor beta2
限制:
Humans
语言:
Ko
期刊:
Journal of the Korean Ophthalmological Society
年:
2005
类型:
Article