Anticancer effect of SN-38 combined with sorafenib on hepatocellular carcinoma in vitro and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 486-492, 2015.
Article
在 Zh
| WPRIM
| ID: wpr-255165
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anticancer effect and its mechanism of SN-38 combined with sorafenib on hepatocellular cancer cell lines HepG-2 and BEL-7402.</p><p><b>METHODS</b>SRB colorimetry was employed to measure the viability of HepG-2 and BEL-7402 cells after the treatment of SN-38 with sorafenib. Propidium iodide flow cytometric assay and DAPI staining were used to evaluate the apoptosis of HCC cells. Western blotting was conducted to detect the expression level of apoptosis-related and DNA damage-related proteins.</p><p><b>RESULTS</b>SRB colorimetry showed the synergistic anticancer activities of SN-38 combined with sorafenib, with a combination index of <0.9. The apoptotic rates of HepG-2 cells in control, 60 nmol/L SN-38, 2.5μmol/L sorafenib and combination groups were 4.25%±2.45%, 28.95%±10.75%, 3.49%±2.49% and 53.19%±11.21%, respectively(P<0.05). Western blotting showed that the combination of these two drugs increased the enzymolysis of PARP, Caspase-8 and Caspase-3, and promoted the expression levels of p53, p21 and γ-H2AX significantly.</p><p><b>CONCLUSION</b>SN-38 and sorafenib have synergistic anticancer activity on hepatocellular carcinoma cells in vitro with the augmentation of apoptosis.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Pharmacology
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Phenylurea Compounds
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Camptothecin
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Histones
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Tumor Suppressor Protein p53
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Poly(ADP-ribose) Polymerases
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Apoptosis
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Niacinamide
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Carcinoma, Hepatocellular
限制:
Humans
语言:
Zh
期刊:
Journal of Zhejiang University. Medical sciences
年:
2015
类型:
Article