Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer / 南方医科大学学报
Journal of Southern Medical University
; (12): 570-572, 2006.
Article
在 Zh
| WPRIM
| ID: wpr-255251
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.</p><p><b>METHODS</b>A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.</p><p><b>RESULTS</b>The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells.</p><p><b>CONCLUSION</b>HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Breast Neoplasms
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RNA, Messenger
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Transfection
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Blotting, Western
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Apoptosis
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Receptor, ErbB-2
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Reverse Transcriptase Polymerase Chain Reaction
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RNA, Small Interfering
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RNA Interference
限制:
Female
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Humans
语言:
Zh
期刊:
Journal of Southern Medical University
年:
2006
类型:
Article