Pretreatment effect of adenosine on activation of NF-kappaB and level of TNF-alpha during myocardial ischemia and reperfusion in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 25-27, 2004.
Article
在 En
| WPRIM
| ID: wpr-270286
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the pretreatment effect of adenosine on NF-kappaB nuclear activity in ischemia/reperfusion myocardium in rats.</p><p><b>METHODS</b>Eighteen healthy male S-D rats were randomly divided into three groups. Group I was the control group. The other two groups were subjected to 30 minutes of ischemia, followed by 2 hours of reperfusion. In group III, adenosine was given 40 microg.kg(-1).min(-1) 30 minutes before coronary artery occlusion. The NF-kappaB in nuclear was extracted and measured with western blot analysis. TNF-alpha levels in myocardium were measured with enzyme-linked immunosorbent assay (ELISA) system. All the data were recorded with mean +/- SEM, differences at the 95% confidence level were considered significant.</p><p><b>RESULTS</b>NF-kappaB activity in the nucleus significantly increased after ischemia/reperfusion and TNF-alpha levels changed. Adenosine significantly inhibited NF-kappaB activity in nucleus, and concomitantly decreased the level of TNF-alpha in myocardium.</p><p><b>CONCLUSIONS</b>Adenosine modulation of NF-kappaB activation may be the cellular molecular mechanism of decreasing of TNF-alpha. The cardioprotective action of adenosine may be involved in the differential modulation of NF-kappaB activation during myocardial ischemia/reperfusion.</p>
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
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Reference Values
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Therapeutics
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Enzyme-Linked Immunosorbent Assay
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Myocardial Reperfusion
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Myocardial Reperfusion Injury
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Random Allocation
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Confidence Intervals
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Adenosine
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Blotting, Western
研究类型:
Diagnostic_studies
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Prognostic_studies
限制:
Animals
语言:
En
期刊:
Chinese Journal of Traumatology
年:
2004
类型:
Article