The expression and clinical significance of miR-203 in pediatric acute leukemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 777-781, 2013.
Article
在 Zh
| WPRIM
| ID: wpr-272115
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the methylation, expression and clinical significance of miR-203 in pediatric acute leukemia.</p><p><b>METHODS</b>The methylation status of miR-203 promoter CpG islands was detected with methylation-specific polymerase chain reaction. The expression of miR-203 was detected by Taqman real- time quantitative polymerase chain reaction. And the clinical significance of miR-203 in pediatric acute leukemia (ALL) was also analyzed.</p><p><b>RESULTS</b>The promoter of miR-203 was unmethylated in all of 31 pediatric acute lymphoblastic leukemia, all of 15 pediatric acute myeloid leukemia (AML) and all of 23 controls. The relative expression levels of miR-203 in controls, pediatric acute leukemia, ALL and AML were 16.93±6.31, 48.97±10.38, 55.88±12.91, 24.28±9.10 respectively. The results indicated that miR-203 was significantly up- regulated in pediatric acute leukemia (P=0.011) and ALL (P=0.009), not in pediatric AML (P=0.514) compared with control. The expression of miR-203 was significantly related with the gender, immunophenotype, chromosome, fusion gene, BCR-ABL, SIL-TAL1 and prednisone experiment in pediatric ALL and the gender, chromosome, fusion gene, SIL-TAL1 in pediatric acute leukemia (P<0.05). And in risk stratification pairwise comparisons, the expression of miR-203 in the medium-risk and high-risk groups appeared significantly different (P=0.022).</p><p><b>CONCLUSION</b>miR-203 may not be regulated with methylation mechanism in pediatric acute leukemia. miR- 203 may be a protooncogene involved in the formation of pediatric acute leukemia and ALL. Further analyses indicated that high expression of miR-203 may be associated with poor prognosis of pediatric ALL and acute leukemia.</p>
全文:
1
索引:
WPRIM
主要主题:
Leukemia
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Leukemia, Myeloid
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Case-Control Studies
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Gene Expression Regulation, Leukemic
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Acute Disease
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Promoter Regions, Genetic
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CpG Islands
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DNA Methylation
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MicroRNAs
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
研究类型:
Observational_studies
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Risk_factors_studies
限制:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
语言:
Zh
期刊:
Chinese Journal of Hematology
年:
2013
类型:
Article