Inhibitory effect of sinomenine on expression of cyclooxygenase-2 in lipopolysaccharide-induced PC-12 cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 900-903, 2004.
Article
在 Zh
| WPRIM
| ID: wpr-272771
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of sinomenine (Sin) on cell proliferation, intracellular expression of cyclooxygenase-2 (COX-2), and production of PGE2 in lipopolysaccharide-induced PC-12 cells, To explore the Sin's mechanism on nerve cell.</p><p><b>METHOD</b>PC-12 cells were cultured with nerve growing factors (NGF), and pretreated with Sin at various concentrations (0, 3 x 10(-6), 30 x 10(-6), 150 x 10(-6) mol x L(-1)) for 2 hours, then with or without stimulation of lipopolysaccharide (LPS). The proliferation activity of PC-12 cells was determined by 3H-TdR incorporation, and the production of PGE2 in culture supernatants of PC-12 cells was detected with competitive ELISA. Expression of COX-2 mRNA in PC-12 cells was analyzed by semi-quantitative RT-PCR, and expression of COX-2 protein was estimated by Western blot method and cellular enzyme immunoassay. Nuclear factor-kappa B (NF-kappaB) activity in whole-cell extract of PC-12 cells was also measured by an ELISA-based method.</p><p><b>RESULT</b>The data showed that Sin down-regulated the expression of COX-2 mRNA and protein, and reduced the production of PGE2 in the LPS-stimulated PC-12 cells which correlated with Sin's concentrations positively. In addition, NF-kappaB activity in LPS-stimulated cells was suppressed significantly by Sin. No inhibition of proliferation of PC-12 cells due to Sin treatment was observed.</p><p><b>CONCLUSION</b>Sin mediates the down-regulation of expression of COX-2 and production of induced PGE2 in PC-12 cells by suppressing the activity of NF-kappaB.</p>
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
/
Plants, Medicinal
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RNA, Messenger
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Dinoprostone
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Down-Regulation
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Chemistry
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Lipopolysaccharides
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NF-kappa B
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PC12 Cells
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Prostaglandin-Endoperoxide Synthases
限制:
Animals
语言:
Zh
期刊:
China Journal of Chinese Materia Medica
年:
2004
类型:
Article