Effect of duration and temperature of sample preservation on the result of peripheral blood CD4+ and CD8+ T lymphocyte count in HIV/AIDS patients / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 129-131, 2004.
Article
在 Zh
| WPRIM
| ID: wpr-281836
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>By analyzing the CD4+ and CD8+ T lymphocyte count of whole blood from HIV/AIDS patients, which were stored at different temperatures for various durations, the authors studied the ideal preserving condition for whole blood and processed, in a purpose of guaranteeing the accuracy of clinical testing of CD4+ and CD8+ T lymphocyte count.</p><p><b>METHODS</b>Blood from 34 HIV carriers/AIDS patients, were kept at 4 degrees C for 2, 24, 48, or 72 h, and tested for CD4+ and CD8+ T lymphocyte count using cytometric analysis. Part of the blood was processed, and kept at degrees C or room temperature for 2, 24, 48, or 72 h, then tested for CD4+ and CD8+ T lymphocyte count. The results were compared statistically in parallel.</p><p><b>RESULTS</b>Whole blood and processed samples preserved at degrees C showed no statistical difference in CD4+ T lymphocyte count among different preserving durations (P greater than 0.05), but CD8+ T lymphocyte counts were significantly different at 72 h (P less than 0.05). Processed samples at 72 h were significantly different in CD4+ T lymphocyte count(P less than 0.05), and significantly different in CD8+ T lymphocyte count at 24 h (P less than 0.05). At room temperature, samples at different duration were not significantly different in CD4+ T lymphocyte count, but significantly different in CD8+ T lymphocyte count at 48 and 72 h (P less than 0.05).</p><p><b>CONCLUSION</b>There were stable results for performing analysis of the CD4+ and CD8+ T lymphocyte count of the anticoagulated blood within 48 h. At room temperature, there were stable results for performing the analysis of CD4+ and CD8+ T lymphocyte count of processed samples within 24 h. Between 24 h and 48 h, although CD4+ count was stable, CD8+ count showed significant changes, so the ratio of CD4 to CD8 changed accordingly.</p>
全文:
1
索引:
WPRIM
主要主题:
Temperature
/
Time Factors
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Blood Preservation
/
HIV Infections
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Acquired Immunodeficiency Syndrome
/
CD8-Positive T-Lymphocytes
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CD4 Lymphocyte Count
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Cell Biology
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Allergy and Immunology
/
Flow Cytometry
限制:
Adult
/
Aged
/
Female
/
Humans
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Male
语言:
Zh
期刊:
Chinese Journal of Experimental and Clinical Virology
年:
2004
类型:
Article