PCR analysis of Yq microdeletions in infertile males, a study from South India / 亚洲男科学杂志(英文版)

ABSTRACT

<p><b>AIM</b>To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.</p><p><b>RESULTS</b>Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.</p><p><b>CONCLUSION</b>The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.</p>