Effect of osthole on bone metabolism in rat femoral tissues in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 561-566, 2013.
Article
在 Zh
| WPRIM
| ID: wpr-285960
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.</p><p><b>METHODS</b>The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.</p><p><b>RESULT</b>The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.</p><p><b>CONCLUSION</b>Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.</p>
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
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In Vitro Techniques
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Calcium
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Rats, Sprague-Dawley
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Coumarins
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Alkaline Phosphatase
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Core Binding Factor Alpha 1 Subunit
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RANK Ligand
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Osteoprotegerin
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Bone Morphogenetic Protein 2
限制:
Animals
语言:
Zh
期刊:
Acta Academiae Medicinae Sinicae
年:
2013
类型:
Article