Expression and mutation of c-kit gene in gastrointestinal stromal tumor / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 461-464, 2002.
Article
在 Zh
| WPRIM
| ID: wpr-301987
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of the expression and mutation of c-kit gene and its relationship with clinical pathology and prognosis of gastrointestinal stromal tumor (GIST).</p><p><b>METHODS</b>Immunohistochemical and PCR-SSCP techniques were used to detect c-kit protein expression and c-kit gene exon 11 mutation in 82 patients with GIST.</p><p><b>RESULTS</b>The positive c-kit protein expression and c-kit gene mutation rates were 97.6% (80/82) and 41.5% (34/82). Correlating the results of these two methods and clinicopathological factors, the c-kit expression and c-kit gene mutation rates were 95.0% (19/20) and 0 in benign GIST, and were 98.4% (61/62), 54.8% (34/62) in malignant GIST. Mutation positive GIST showed higher frequency of adjacent tissue invasion, metastasis and recurrence as compared with mutation negative ones.</p><p><b>CONCLUSION</b>c-kit protein is an important diagnostic marker of gastrointestinal stromal tumor. c-kit gene mutation may play a significant role in the pathogenesis of GIST and also may be a prognostic marker.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Prognosis
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational
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Proto-Oncogene Proteins c-kit
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Diagnosis
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Gastrointestinal Neoplasms
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Genetics
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Mutation
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Neoplasm Metastasis
研究类型:
Diagnostic_studies
/
Prognostic_studies
限制:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
语言:
Zh
期刊:
Chinese Journal of Oncology
年:
2002
类型:
Article