The protective function and mechanism of notoginsenoside Rb1 against hypoxia hypercapnia-induced pulmonary vasoconstriction / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 1380-1384, 2012.
Article
在 Zh
| WPRIM
| ID: wpr-309349
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective function and mechanism of notoginsenoside Rb1 against hypoxia hypercapnia-induced pulmonary vasoconstriction (HHPV).</p><p><b>METHODS</b>The pulmonary artery smooth muscle cells of healthy male SD rats were primarily cultured and the second to the fifth subcultured cells were incubated with 8, 40, and 100 mg/L notoginsenoside Rb1 respectively under the hypoxia-hypercapnia condition (1% O2 and 6% CO2). The cells were harvested for 24 h. The phosphated extracellular signal-regulated kinase (p-ERK) protein expression of the cells was detected by Western blot. The mRNA expressions of ERK1 and ERK2 were detected using half quantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The expression of p-ERK protein, the mRNA expressions of ERK1 and ERK2 were weakly positive in the control group. Their expressions in the hypoxia-hypercapnia group were obviously enhanced (P < 0.01). After intervention of Rb1 at different concentrations, their expressions were obviously lowered (P < 0.05, P < 0.01) in a dose-dependent manner. The optimal effects were obtained at the dose of 100 mg/L. The expression of p-ERK protein was significantly positively correlated with mRNA expressions of ERK1 and ERK2 in notoginsenoside Rbl-treated groups (r = 0.500, P < 0.01; r = 0.977, P < 0.01).</p><p><b>CONCLUSIONS</b>ERK1/2 pathway might play a role in the rat HHPV. Notoginsenoside Rb, could alleviate HHPV by inhibiting the ERK1/2 pathway.</p>
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
/
Pulmonary Artery
/
Vasoconstriction
/
Cell Hypoxia
/
Cells, Cultured
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Rats, Sprague-Dawley
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Mitogen-Activated Protein Kinase 1
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Cell Biology
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Myocytes, Smooth Muscle
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Ginsenosides
限制:
Animals
语言:
Zh
期刊:
Chinese Journal of Integrated Traditional and Western Medicine
年:
2012
类型:
Article