Effects of hypoxia inducible factors-1alpha on autophagy and invasion of trophoblasts / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
; : 73-80, 2012.
Article
在 En
| WPRIM
| ID: wpr-30941
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-1alpha on the cell death, autophagy, and invasion of trophoblasts.METHODS:
To understand the effect of HIF-1alpha, we inhibited HIF-1alpha using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-1alpha. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy.RESULTS:
There was no difference in cell death through the inhibition of HIF-1alpha expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-1alpha expression by siRNA. These experimental results mean that HIF-1alpha genes regulate the invasive ability of trophoblasts by increasing autophagy.CONCLUSION:
This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-1alpha and autophagy.Key words