Effects of erigeron injection on renal interstitial fibrosis in rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 142-145, 2008.
Article
在 Zh
| WPRIM
| ID: wpr-315207
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and mechanism of Erigeron Injection (EI) on renal interstitial fibrosis in rats.</p><p><b>METHODS</b>Unilateral ureteral obstruction (UUO) model rats were taken as the subject of study. Thirty-six Sprague-Dawley rats were randomly divided into the control group (A), the UUO model group (B) and the treatment group (C) treated with intraperitoneal injection of EI 5 mL/kg per day from 24 h before to 9 days after the operation. On the 10th day of UUO, rats were killed and their kidneys were processed to paraffin sections with HE, PAS and picro-sirius-red staining. The pathological change of renal tubular interstitial tissue and relative cortical/interstitial volume (C/I) as well as the relative content of collagen (RC) were observed by light microscope. The expression of transforming growth factor beta1 (TGF-beta1), alpha-smooth muscle actin (alpha-SMA) and collagen I in the renal mesenchyma were examined by immunohistochemistry.</p><p><b>RESULTS</b>Marked renal interstitial fibrosis changes were found in Group B and C, but the changes were milder in Group C. C/I and RC were higher in Groups B and C as compared with those in Group A (P < 0.01), but they were much lower in Group C than in Group B (P < 0.01). The expression of TGF-beta1, alpha-SMA and collagen I were higher in Group B and C than those in Group A (P < 0.05), but they were lower in Group C than in Group B (P < 0.05).</p><p><b>CONCLUSION</b>EI could ameliorate renal interstitial fibrosis in rats, which might be partially realized by down-regulating the expression of TGF-beta1 to prevent the renal epithelial cell differentiation and reducing the synthesis of collagen I.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
/
Ureteral Obstruction
/
Fibrosis
/
Drugs, Chinese Herbal
/
Immunohistochemistry
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Random Allocation
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Chemistry
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Transforming Growth Factor beta
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Rats, Sprague-Dawley
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Therapeutic Uses
研究类型:
Prognostic_studies
限制:
Animals
语言:
Zh
期刊:
Chinese Journal of Integrated Traditional and Western Medicine
年:
2008
类型:
Article