Study on tumor necrosis factor-alpha and interleukin 2 in rat skin allograft / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To elucidate the histocompatibility of tissue engineered rat skin through studying the effect of TNF-alpha and IL-2 in immunological rejection after rat skin allograft.</p><p><b>METHODS</b>Tissue engineered skin that the basic materials were taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-alpha and IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.</p><p><b>RESULTS</b>The expressions of TNF-alpha and IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.</p><p><b>CONCLUSION</b>The expressions of TNF-alpha and IL-2 and the immunological rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn't arose obvious immunological rejections.</p>