Molecular epidemiology of diarrhea among children with Norwalk-like virus in a hospital of Guangzhou in autumn and winter / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 525-528, 2005.
Article
在 Zh
| WPRIM
| ID: wpr-331843
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the epidemiological characteristics of Norwalk-like virus (NLVs) infection in children with diarrhea and to study the genotype and predominant cluster at a hospital in Guangzhou city.</p><p><b>METHODS</b>Fecal specimens from 358 children with acute gastroenteritis from October 2003 to January 2004 and information about the cases were collected. NLVs was detected from the specimens by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products were purified and sequenced.</p><p><b>RESULTS</b>Forty-two positive specimens were detected from the 358 fecal specimen with a positive rate of 11.73% (42/358). Of these, 40 specimens were obtained from infants younger than 3 years of age. The youngest infant infected with NLVs in this study was only 25 days. The positive rate in November (17.27%) was the highest. Eleven positive PCR products were selected and sequenced. Nucleotide sequence analysis revealed that 11 strains all belong to genogroup II (G II), and of these, 5 strains belonged to G II-3 cluster, with another 5 strains belonged to G II-4 cluster. However, one strain with its cluster could not be determined.</p><p><b>CONCLUSION</b>NLVs served as one of the important pathogens causing sporadic acute gastroenteritis among children at a hospital in Guangzhou. The predominant strains were identified as G II-3 and G II-4 cluster.</p>
全文:
1
索引:
WPRIM
主要主题:
Phylogeny
/
Physiology
/
Seasons
/
Virology
/
RNA, Viral
/
Sequence Homology, Nucleic Acid
/
China
/
Epidemiology
/
Classification
/
Sex Distribution
研究类型:
Prognostic_studies
/
Screening_studies
限制:
Child, preschool
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Female
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Humans
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Infant
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Male
/
Newborn
国家/地区名称主题:
Asia
语言:
Zh
期刊:
Chinese Journal of Epidemiology
年:
2005
类型:
Article