Vimentin significantly promoted gallbladder carcinoma metastasis / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 4236-4244, 2011.
Article
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| WPRIM
| ID: wpr-333580
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The precise molecular mechanisms underlying the gallbladder carcinoma (GBC) metastasis has not been fully elucidated.</p><p><b>METHODS</b>In the present study, metastasis-associated proteins were identified by comparative proteomic analysis. The functional study of the candidate protein vimentin was further investigated. First, a pair of higher and lower metastatic sublines (termed GBC-SD/M3 and GBC-SD, respectively), originated from the same parental cell line, was screened by spontaneous tumorigenicity and metastasis in vivo in animal study and further characterized by metastatic phenotypes analysis in vitro. Subsequently, a proteomic approach comprised two-dimensional gel electrophoresis analysis and mass spectroscopy was used to identify and compare the protein expression patterns between higher metastatic GBC-SD/M3 and lower metastatic GBC-SD cell lines. Then twenty-six proteins were identified.</p><p><b>RESULTS</b>Among the 26 proteins identified, fourteen proteins were up-regulated and 12 proteins were down-regulated in GBC-SD/M3. Vimentin was identified and found to be overexpressed in GBC-SD/M3 as compared with GBC-SD. This result was further confirmed by quantitative PCR and Western blotting analysis. Furthermore, the cell migration and invasion potency of GBC-SD/M3 in vitro was remarkably suppressed after small interference RNA-mediated knockdown of vimentin. Moreover, immunoblot and immunohistochemical analysis on 12 human GBC specimens showed consistently increased vimentin expression in metastases compared with primary tumors.</p><p><b>CONCLUSION</b>Tumor vimentin level may reflect the pathological progression in some GBC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of GBC patients with metastases.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Physiology
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Vimentin
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Immunohistochemistry
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Electrophoresis, Gel, Two-Dimensional
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Cell Movement
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Blotting, Western
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Reverse Transcriptase Polymerase Chain Reaction
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RNA Interference
限制:
Animals
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Humans
语言:
En
期刊:
Chinese Medical Journal
年:
2011
类型:
Article