Effects of RNA interference against aggrecanase 1 gene on extracellular matrix metabolism of cultured chondrocytes in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1766-1769, 2009.
Article
在 Zh
| WPRIM
| ID: wpr-336091
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of RNA interference (RNAi)-mediated aggrecanase-1 gene silencing on extracellular matrix metabolism of cultured rat costochondral chondrocytes.</p><p><b>METHODS</b>Rat costochondral chondrocyte monolayers were obtained by microdissection and digestion. The growth and morphological changes of the chondrocytes were observed after RNAi of aggrecanase-1 gene. The mRNA expression of aggrecanase-1 was detected by RT-PCR method, and aggrecan content was determined by Western blotting.</p><p><b>RESULTS</b>The specific inhibition of aggrecanase-1 by RNAi produced no adverse effect on the morphology and growth of the chondrocytes. The mRNA of aggrecanase-1 decreased and aggrecan content increased significantly after transfection of the chondrocytes.</p><p><b>CONCLUSION</b>Inhibition of aggrecanase-1 decreases aggrecan degradation in cultured rat chondrocytes. RNAi technique can be a useful means for studying extracellular matrix metabolism in the cartilage.</p>
全文:
1
索引:
WPRIM
主要主题:
Procollagen N-Endopeptidase
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RNA, Messenger
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Transfection
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Cells, Cultured
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Rats, Sprague-Dawley
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Chondrocytes
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Cell Biology
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RNA Interference
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ADAM Proteins
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Extracellular Matrix
限制:
Animals
语言:
Zh
期刊:
Journal of Southern Medical University
年:
2009
类型:
Article