A method for simultaneous assay of propulsion and absorption in small intestine / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To develop a method for simultaneous assay of propulsion and absorption in small intestine.</p><p><b>METHODS</b>The mice were administrated through gastric tube with mixed reagents containing 0.12% phenol red, D-xylose (1.25%, 2.5% and 5%) and 15% gelatin. The influence of phenol red on D-xylose absorption and the influence of D-xylose on small intestine propulsion rate were investigated by measuring serum concentration of D-xylose with phloroglucinol method.</p><p><b>RESULTS</b>At 10 min, no significant difference was found between 5% D-xylose mixed reagent group and 5% D-xylose control. At 15 min, small intestine propulsion rate in 5% D-xylose mixed reagent group, but not in 2.5% and 1.25% D-xylose mixed reagent groups, was significantly higher than in phenol red control (P<0.05).</p><p><b>CONCLUSION</b>Gastric administration of mixed reagent containing 0.12% phenol red, 5% D-xylose and 15% gelatin can simultaneously assay propulsion and absorption of small intestine in mice.</p>