Determination of icaritin in rat plasma by HPLC-MS/MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 1140-1144, 2009.
Article
在 Zh
| WPRIM
| ID: wpr-344042
Responsible library:
WPRO
ABSTRACT
The paper is to report the development of a high-performance liquid chromatographic/tandem mass spectrometry (HPLC-MS/MS) method for the determination of icaritin (ICT) in rat plasma. After precipitated with acetonitrile from the plasma, ICT was isolated chromatographically on a Dikma C18 column. The mobile phase consisted of acetonitrile-water-acetic acid (72 : 28 : 1.5, v/v/v). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 387 --> m/z 313 and m/z 331 --> m/z 315 were used to quantify ICT and the internal standard, respectively. The linear calibration curve was obtained in the concentration range of 2.5-1,000 ng x mL(-1). The lower limit of quantification was 2.5 ng x mL(-1). The inter- and intra-day precision (RSD) were less than 9.63%, and the accuracy (relative error) was within +/-7.42%. The method was proved to be suitable for the pharmacokinetics of ICT, which offers advantages of high sensitivity and selectivity.
全文:
1
索引:
WPRIM
主要主题:
Plants, Medicinal
/
Flavonoids
/
Blood
/
Pharmacokinetics
/
Chemistry
/
Administration, Oral
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Rats, Wistar
研究类型:
Diagnostic_studies
限制:
Animals
语言:
Zh
期刊:
Acta Pharmaceutica Sinica
年:
2009
类型:
Article