Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 436-439, 2005.
Article
在 Zh
| WPRIM
| ID: wpr-348781
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.</p><p><b>METHODS</b>Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.</p><p><b>RESULTS</b>10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.</p><p><b>CONCLUSION</b>The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Electrophoresis, Gel, Two-Dimensional
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Carcinoma, Hepatocellular
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Phosphotyrosine
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Cell Line, Tumor
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Liver Neoplasms
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Metabolism
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Neoplasm Metastasis
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Neoplasm Proteins
限制:
Humans
语言:
Zh
期刊:
Chinese Journal of Hepatology
年:
2005
类型:
Article