Identification of metastasis-associated proteins of ovarian cancer by proteomics / 中华病理学杂志
Chinese Journal of Pathology
; (12): 814-818, 2007.
Article
在 Zh
| WPRIM
| ID: wpr-350004
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.</p><p><b>METHODS</b>The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.</p><p><b>RESULTS</b>Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.</p><p><b>CONCLUSION</b>The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.</p>
全文:
1
索引:
WPRIM
主要主题:
Ovarian Neoplasms
/
Pathology
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Electrophoresis, Gel, Two-Dimensional
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Gene Expression Regulation, Neoplastic
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Proteomics
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Cell Line, Tumor
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Metabolism
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Neoplasm Metastasis
研究类型:
Diagnostic_studies
/
Prognostic_studies
限制:
Female
/
Humans
语言:
Zh
期刊:
Chinese Journal of Pathology
年:
2007
类型:
Article