Determination of acetylharpagide in Ajuga decumbens by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 289-290, 2006.
Article
在 Zh
| WPRIM
| ID: wpr-350958
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.</p><p><b>METHOD</b>The chromatographic conditions were as follows a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (1585), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.</p><p><b>RESULT</b>Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.</p><p><b>CONCLUSION</b>The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.</p>
全文:
1
索引:
WPRIM
主要主题:
Plants, Medicinal
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Pyrans
/
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
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Chromatography, High Pressure Liquid
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Sensitivity and Specificity
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Ajuga
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Methods
研究类型:
Diagnostic_studies
语言:
Zh
期刊:
China Journal of Chinese Materia Medica
年:
2006
类型:
Article