Preparation and characterization of a directly labeled mouse anti-human CD14 monoclonal antibody ZCH-2F9-FITC / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 167-171, 2005.
Article
在 Zh
| WPRIM
| ID: wpr-353225
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare fluorescein isothiocyanate (FITC) directly conjugated to monoclonal antibody (McAb) anti-human CD14, ZCH-7-2F9 (2F9-FITC).</p><p><b>METHODS</b>After generation and purification, the purity and the murine immunoglobulin subtype of the antibody were evaluated with SDS-PAGE and multicolor flow cytometry (FCM). 2F9 McAb was directly labeled with FITC through modified Marsshall's method and the positive rate of the 2F9-FITC on different types of leukemic cells were compared with the standard CD14-FITC by FCM.</p><p><b>RESULT</b>A large quantity of purified 2F9 McAb was prepared. The subtype of 2F9 was murine IgG1kappa. 2F9-FITC was successfully manufactured with A295/A280 ratio of 0.44. The positive cell percentages of 2F9-FITC and CD14-FITC on the monocytes were 84.50% and 90.08%, respectively, while those on lymphocytes were only 0.52% and 1.01%. There was no significant difference between the CD14 expressions with 2F9-FITC and CD14-FITC on each type of leukemia (n=23, t=0.922, P=0.367).</p><p><b>CONCLUSION</b>2F9-FITC has been successfully prepared and it can be applied in diagnosis and differentiation of monoblastic leukemias.</p>
全文:
1
索引:
WPRIM
主要主题:
Pathology
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Monocytes
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Leukemia, Myeloid, Acute
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Leukemia, Lymphoid
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Cells, Cultured
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Fluorescent Antibody Technique
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Fluorescein-5-isothiocyanate
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Lipopolysaccharide Receptors
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Cell Biology
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Allergy and Immunology
限制:
Animals
/
Humans
语言:
Zh
期刊:
Journal of Zhejiang University. Medical sciences
年:
2005
类型:
Article